Figure 3

Figure 3

Somatostatin 1.1 does not contribute to the bout rate nor to the ratio of forward bouts and turns during exploration. (a) Experimental paradigm used to record spontaneous slow swim in 5-dpf zebrafish larvae over 5 min at 100 Hz after 10 min of acclimation. (b) The kinematic analysis relies on the measure of the tail angle α over time as shown for 3 different larvae. (c) Bout rate is similar for sst1.1^icm40/icm40 mutant larvae and control WT siblings (Grey line: mean ± SEM. 0.91 ± 0.04 Hz, N = 58 larvae; 0.95 ± 0.04 Hz, N = 62 larvae; Wald χ²(1) = 0.084, p = 0.771). Black lines: medians. (d) Probability density function of the absolute maximal bend amplitude for all bouts of sst1.1^icm40/icm40 mutant larvae (red) versus WT siblings (blue). Forward swims and routine turns were sorted with a 25-degree threshold (dashed line). (e) The ratio of forward swims (orange) and routine turns (green) is similar for sst1.1^icm40/icm40 mutant larvae and WT siblings.