Figure 2

Figure 2

Characterization of zip9-mutant phenotype. (A) Percent of viable eggs ovulated by zip9^-/- and zip9^+/+ females 2 h post fertilization (hpf); n = 13–14 clutches. (B) Representative image of zip9^+/+ and zip9^-/- embryos at 4 hpf and 1 and 3 days post fertilization (dpf). Scale bars: 500 µm. Arrows donate the yolk sac in WT and mutants (3 dpf) and asterisks donate edema in zip9^-/- larvae (3 dpf). (C) Chorion diameter of eggs spawned by zip9^-/- and zip9^+/+ females; n = 40. (D) Yolk volume of 3 dpf zip9^-/- and zip9^+/+ larvae; n = 16–18. (E) Length of zip9^-/- and zip9^+/+ larvae without exogenous feeding between 3–10 dpf; n = 10–21. (F) Incidence of pericardial/yolk sac edema in 6 dpf zip9^-/- and zip9^+/+ larvae; n = 5 clutches. All data represents means ± SEM. Significance was determined by Welch's t-test (*, P < 0.05; **, P < 0.001; ***, P < 0.0001).