Figure 5

Figure 5

Electrical stimulation apparatus made to create a low amplitude, consistent noxious stimulus. (A) The box was 3D printed using free software (TinkerCad) with five chambers. The first and last chamber held a gold pin for the electrical current. The middle three chambers were for zebrafish larvae. The chambers were separated by a semi-permeable cloth membrane (dotted lines). This allowed liquid and, therefore, current, to flow but did not allow the larvae to move between chambers. (B) The apparatus (red arrowhead) attached to the Grass stimulator (black arrowhead) was placed within a Noldus DanioVision behavior box (black arrow) and a video of their movement was recorded (red arrow). (C) The zebrafish larvae moved 93.3% ± 6.1% of the time to the ELEC stimulus. n = 5 replicates with 12 zebrafish per replicate. (D, E) Two separate boxes were fabricated and there was no significant difference between the µAmps generated in the lanes of the two boxes. n = 3 replicates.