Fig. 2

Fig. 2

a Schematic indicating the two waves of T cell development in zebrafish, operationally referred to as larval and adult phases; the larval phase depends on the activity of the ikzf1 transcripton factor^28,46. In the forward genetic screen, fish were analyzed at 5 dpf. b Expression patterns of selected genes associated with hematopoietic development in 5 dpf G2 embryos. Levels were determined by qPCR and normalized to the levels of actb1 (n = 4; mean; whiskers represent maximum and minimum values; individual data points are indicated). c Adult lymphopoiesis fails in mutant fish. Flow cytometric analyses of whole kidney marrow (WKM) cells⁴⁷ of wild-type and dnmt1 mutants, both transgenic for an ikzf1-EGFP reporter^27,28 (left panels). Circles denote different blood cell populations in adult wild-type fish: red, erythrocytes; blue, lymphocytes; magenta, precursors; green, myelomonocytes. Percentages (mean ± SD) of each population in WKM preparations are indicated (n = 4, for both genotypes; left panels). Analysis of EGFP-positive cells in the lymphocyte gates; percentages of positive cells are indicated in the histograms (n = 4, for both genotypes; middle panel), and enumerated (right panels). FSC, forward light scatter; SSC, side light scatter. Source data are provided as Source Data file. For b, c, unpaired two-tailed t test with Welch´s for unequal variance and Bonferroni´s correction for multiple tests.