IMAGE

Figure 5—figure supplement 1.

ID: ZDB-IMAGE-201003-176
Publication: Boezio et al., 2020 - Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract
All Figures: Figures for Boezio et al., 2020

Image

Figure Caption

Figure 5—figure supplement 1. <italic>alk5</italic> endothelial-specific overexpression is sufficient to restore OFT wall formation and function in <italic>alk5</italic> mutants.

(A–A’’) Ventral view of 120 hpf Tg(fli1a:alk5b-mScarlet) alk5+/+ larvae, showing the restricted expression of the transgene in the ECs. Boxed area is shown in A’ and A’’; dotted lines outline the OFT; arrowheads, SMCs. (B, C) Brightfield images of 9 dpf alk5+/+ (B) and alk5-/- animals carrying the EC-specific rescue transgene (Tg,C) (11/108 alk5 mutants analyzed inflated their swim bladder). Black arrowheads, swim bladder; pink arrowhead, deformation of the head. (D) Percentage of SMCs surrounded by Eln2 immunostaining (per sagittal plane) at 75 hpf. (E) Percentage of EdU⁺ SMCs in 72 hpf alk5+/+, alk5-/-, and Tg(fli1a:alk5b-mScarlet) alk5-/- animals. (D, E) Means ± SD; p value from Kruskal-Wallis tests, compared with the first column (alk5+/+). Scale bars: (A) 50 μm; (A’, A’’) 20 μm; (B, C) 200 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife