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Figure 3—figure supplement 1. Energy charge in sphere stage embryos as well as internalization and movement speed of deep cells are not affected in mondoa morphants.

(A) Energy charge values [([ATP] + 0.5 [ADP])/([ATP] + [ADP] + [AMP])] at sphere stage as calculated for uninjected embryos, embryos injected with mondoa-mis and embryos injected with mondoa-mo showed no significant differences between the three conditions (One-way-ANOVA, p=0.4287). (B) Still images of internalization of cells in control injected embryo (Video 6). Traces of single cells determined by automated cell nucleus tracking are highlighted (timescale in hpf: red to yellow). (C–E) Movement speed of deep cells is not altered in mondoa-mis (C, E) and mondoa-mo injected embryos (D, E). p=0.6743.

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