ZFIN Image: Steindal et al., 2020, FIGURE 3

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Figure Caption

FIGURE 3

Light-induced phase shifts in response to monochromatic light-pulses in per1-luciferase zebrafish cells. (A) Cells were entrained for 3 days on a 12:12 LD cycle, before they were given a variety of monochromatic light pulses at ZT21, denoted by black arrow. The cells were kept in constant dark over two subsequent days. (B) Light pulses cause a phase advance in hours, determined at the half maximum between peak and trough vs. no LP controls. A two-way ANOVA (time, wavelength) was used to determine significant variation between samples. Amplitude and baseline were detrended using BioDare2 (Zielinski et al., 2014), and a Tukey post-test was used to determine significance in phase shift relative to DD control. ^∗p < 0.01 and ^∗∗p < 0.001 (n = 8).

Acknowledgments

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