Regional responses to a complex auditory stimulus train. Auditory responsive ROIs (a) in WT and fmr1−/− larvae (equivalent to five larvae). Z-scored activity trace (b) of all responsive ROIs (mean ± SD) with stimulus timing and amplitude represented (bottom). For each of six functional clusters across four brain regions (c–h), the distribution of responsive ROIs (left), percent of all ROIs belonging to the cluster, mean response strength to each of eight stimulus amplitudes (top right), and mean z-scored activity trace during the first amplitude ramp and first twelve discrete amplitudes (bottom right). Spheres (c–h, left) are color coded to genotype. Amplitudes are represented in decibels (dB) from full volume. ON, octavolateralis nucleus; Teg, tegmentum; TS, torus semicircularis; Th, thalamus. P values ≤ 0.1 are shown
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