FIGURE 2

FIGURE 2

Light induction by monochromatic light-pulses in PAC2 and clockDN cell lines. Zebrafish cell lines were maintained on a 12:12 light-dark cycle before being given a 3-hour light pulse of varying wavelengths. Black and white bars represent clockDN cell line expression, whilst solid grey represent PAC2 cell line expression. (A) Light induction of cry1a. (B) Light induction of per2. (C) Light induction of 6–4 Photolyase. (A–C) is plotted as fold induction relative to dark control. (D) Dark controls (black bars) vs. IR monochromatic light pulse (striped bars) plotted relative to lowest expressed gene (PAC2 per2 DD) in clockDN and PAC2 cells. Significance was addressed with an one-way ANOVA (α = 0.05) for each light-pulse, cell line and gene, followed by a Bonferroni post-test. All light pulses give a significant increase of p < 0.05 unless marked on the graph (n = 3).