Figure 6—figure supplement 3.

Figure 6—figure supplement 3.

Given the similarities between Tg(her4.3:EGFP)⁺ EGCs and Tg(her4.3:EGFP)⁺ RGCs, we propose that like RGCs, EGCs may exist in two forms: Tg(her4.3:EGFP)⁺ quiescent EGCs (qEGCs) and Tg(her4.3:EGFP)⁺ activated EGC (aEGCs), the latter of which are proliferative and can take up EdU in our experiments (indicated in blue). We suggest that aEGCs are a self-renewing population, which may also revert to the quiescent state. The proliferative aEGC population can give rise to enteric neuronal progenitors (eNP; cells committed to the neurogenic lineage), which can retain EdU but are Tg(her4.3:EGFP)^- and will not yet express HuC/D. These cells would correspond to the Cherry⁺GFP^-HuC/D^-EdU⁺ cells quantified in Figure 6I, which increase during the EdU labelling period of our experiments. Finally, neural progenitors undergo full neuronal differentiation (eN), can be detected with HuC/D and are also EdU⁺ in our experiments. These cells correspond to the Cherry⁺GFP^-HuC/D⁺EdU⁺ quantified in Figure 6H, which also increase during the course of our EdU labelling experiments.