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Fig. 1 Fig. 1. 5 dpf zebrafish larva (wildtype) stained for cartilage (blue) and bone (red). A total of nine parameters for cartilage were assessed as shown in panels A (ventral view) and B (lateral view): 1 Length ceratohyal to Meckel's cartilage, 2 Length ceratohyal to posterior end of the head, 3 Width at Meckel's cartilage and palatoquadrate joint, 4 Width at ceratohyal and palatoquadrate joint, 5 Length of ceratohyal superior, 6 Length of ceratohyal inferior, 7 Length of ethmoid plate, 8 Lateral length of Meckel's cartilage. The long central arrow indicates total head length (THL). Scale bar is 200 ?m. C: The nine mineralized parameters scored for presence or absence (ventral view): 1) Parasphenoid, 2) Otoliths (4 in total), 3) Cleithrum, 4) Notochordal sheath, 5) Ceratobranchial 5, 6) teeth (on ceratobranchial 5), 7) opercles, 8) Branchiostegal ray 1, 9) Entopterygoid bone. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Reprinted from Mechanisms of Development, 163, Gebuijs, I.G.E., Metz, J.R., Zethof, J., Carels, C.E.L., Wagener, F.A.D.T.G., Von den Hoff, J.W., The anti-epileptic drug valproic acid causes malformations in the developing craniofacial skeleton of zebrafish larvae, 103632, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.