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Figure 6 - figure supplement 1

ID: ZDB-IMAGE-200916-25
Publication: Ichino et al., 2020 - Building the vertebrate codex using the gene breaking protein trap library
All Figures: Figures for Ichino et al., 2020

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Figure Caption

Figure 6 - figure supplement 1 GBT protein traps illuminate diverse subcellular protein localizations.

(A–B) Confocal images demonstrating patterns of subcellular localization seen in muscle with strong banding in GBT0374 (candidate gene = unannotated transcript) (A) and large, diffuse puncta in GBT0708 (candidate gene = adam15) (B). Scale bars = 10 µm. (C) Confocal image of GBT0908 with ubiquitous, cytoplasmic expression. Note apical enrichment in enterocytes. (D) Confocal image of GBT0743 (candidate gene = pole4) with variegated expression in the liver. (E–F) Confocal images of gut expression with cytoplasmic, pan-enterocyte labeling in GBT0361 (E) in contrast with endomembrane puncta and enrichment of mRFP signal in a subset of enterocytes in GBT0775 (candidate gene = cd83) (F).

Acknowledgments

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