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ZDB-IMAGE-200910-34
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Figures for Chetty et al., 2020
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Fig. 3 Loss of Ets1 function impairs vasculogenesis in Etv2-deficient embryos. (A–D) Confocal micrographs of 28 hpf Tg(kdrl:GFP; kdrl:nls-mCherry) wild-type, ets1−/−, etv2 MO (0.25 ​ng) and ets1−/−; etv2 MO (0.25 ​ng) zebrafish embryos (trunk region). (E) Quantitative analysis of endothelial cell number in the axial vessels of embryos in A-D by cell counts in Imaris. (F) FACS analysis of GFP ​+ ​endothelial cells in 28 hpf embryos. Note the decrease in endothelial cell number between etv2 MO and ets1−/−; etv2 MO embryos (E,F). Lateral views; anterior is to the left. ∗P ​< ​0.05, ∗∗∗P ​< ​0.001; n.s – not significant; error bars represent ​± ​SEM.

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Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.