ZFIN Image: Chetty et al., 2020, Fig. 3

Image

Figure Caption

Fig. 3 Loss of Ets1 function impairs vasculogenesis in Etv2-deficient embryos. (A–D) Confocal micrographs of 28 hpf Tg(kdrl:GFP; kdrl:nls-mCherry) wild-type, ets1−/−, etv2 MO (0.25 ng) and ets1−/−; etv2 MO (0.25 ng) zebrafish embryos (trunk region). (E) Quantitative analysis of endothelial cell number in the axial vessels of embryos in A-D by cell counts in Imaris. (F) FACS analysis of GFP + endothelial cells in 28 hpf embryos. Note the decrease in endothelial cell number between etv2 MO and ets1−/−; etv2 MO embryos (E,F). Lateral views; anterior is to the left. ∗P < 0.05, ∗∗∗P < 0.001; n.s – not significant; error bars represent ± SEM.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.