Fig. 3.

Fig. 3.

Observation of multiple tumor foci in p53/nf1/suz12-deficient zebrafish lines. (A) Fluorescence microscopy image of an adult p53^+/−, nf1b^−/−, nf1a^+/−, suz12a^+/−, suz12b^+/− fish bearing two independent GFP-positive tumors in abdominal and anal locations. (B) Proportion of fish with multiple lesions in different suz12-mutant populations with additional p53^+/−, nf1b^−/−, nf1a^+/− or p53^+/−, nf1b^−/−, nf1a^+/+ backgrounds. Data are mean±s.d. of populations with any combination of three out of four (suz12^{+/−/−/−}), two out of four (suz12^+/+/−/−), or one out of four (suz12^+/+/+/−) suz12 alleles knocked out. The total number of fish analyzed is indicated (n). P-values were calculated using Student's t-tests. (C) Representative HE-stained sections of p53/nf1/suz12-deficient fish with two separate tumor foci. Squares indicate areas shown in magnified images below.