Figure 2

Figure 2

DIO leads to increased fasting blood glucose levels and to liver steatosis in zebrafish. (A) Fasting blood glucose measurements at week 4 in CTRL and DIO-treated fish. (B) Zebrafish liver pictures highlighting the increased size and yellowish color of liver in DIO-treated fish compared to CTRL. (C), (E) Liver sections stained with Oil Red O in two control fish showing no lipid accumulation. (D), (F) Liver sections stained with Oil Red O in two DIO-treated fish showing different levels of lipid accumulation (red color) and highlighting liver steatosis. Nuclei were stained with hematoxylin (C–F). These pictures are representative of 3 fish studied. n = number of fish. Student's t-test: ***p < 0.001. Error bars correspond to standard error of the mean (SEM). Scale bar: 1.5 mm (A); 250 μm for lower magnification pictures, 50 μm for higher magnification ones (C–F).