Fig. 5

Fig. 5

The effect of sulforaphane pretreatment on the survival of zebrafish larvae after exposure to H₂O₂.

(A) A schematic illustration of the experiment. Larvae were treated with 2

mM of H₂O₂ at 4 dpf after 12-h pretreatment with 40 μM sulforaphane (SF).

(B, C) The survival rate of keap1a^it302 and keap1b^it308 larvae.

(D) The fold increase in the expression of Nrf2 target genes in keap1a- and keap1b-homozygous knockout and wild-type larvae with sulforaphane treatment. Larvae at 5 dpf were treated with or without sulforaphane at the indicated concentration for 6 h, and the expression of prdx1 and gstp1 was analyzed by qPCR. The expression of untreated larvae was normalized to 1 (arrowheads, white dotted lines). Each experiment was conducted at least three times with duplicate samples. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001). ns, not significant.