Fig. 4

Fig. 4

Survival assays ofkeap1a-andkeap1b-knockout larvae against H₂O₂.

(A) The survival rate of keap1a^it302 and keap1b^it308 larvae after exposure to H₂O₂. At 4 dpf, larvae were exposed to 2 mM of H₂O₂, and the survival was observed every 12 h until 9 dpf. Tested numbers were as follows: keap1a^it302 (WT n = 53, +/– n = 99, −/− n = 46), keap1b^it308 (WT n = 59, +/– n = 107, −/− n = 45). Data from four independent experiments were combined.

(B) The survival rate of keap1a^it302;nfe2l2a^fh318 and keap1b^it308;nfe2l2a^fh318 compound mutant larvae. Tested numbers were follows: keap1a^it302;nfe2l2a^fh318 (−/−;WT n = 16, −/−; +/m n = 25, −/−;m/m n = 17, –/+; WT n = 31, –/+; m/+ n = 94, –/+; m/m n = 45, WT; WT n = 16, WT; +/m n = 38, WT; m/m n = 21), keap1b^it308;nfe2l2a^fh318 (−/−;WT n = 22, −/−; +/m n = 31, −/−;m/m n = 18, –/+; WT n = 32, –/+; m/+ n = 79, –/+; m/m n = 37, WT; WT n = 37, WT; +/m n = 50, WT; m/m n = 26). Data from four independent experiments were combined. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001).