Figure 1.

Figure 1.

tmem216 was widely expressed in zebrafish. In situ hybridization with tmem216 sense and antisense probes was performed on cryosections of 3- and 7-dpf zebrafish larvae. (A) Antisense probe labeling of longitudinal sections of a 3-dpf larva. tmem216 was widely expressed throughout the zebrafish, including the muscle, pronephros, brain, intestine, and liver. (B, C) Antisense probe labeling of 3-dpf zebrafish head. tmem216 was widely expressed throughout the brain and neural retina. (D) Antisense probe labeling of 7-dpf zebrafish retina. tmem216 expression persisted throughout the neural retina at 7-dpf. (E–H) Sense probe labeling of 3- and 7-dpf zebrafish cryosections. (I) RT-PCR of wildtype fish showing TMEM216 expression in newly fertilized eggs, 7-dpf larva, and 8-mpf eye, brain, and skeletal muscle. Scale bar in H: 200 µm for A, B, E, and F; 15.5 µm for C, D, G, and H.