Figure 3-S1

Figure 3-S1

(a) Frequency of liver infiltration at 8–10 hr after brain-LPS injection showed that only Bay 11–7082 (1 µM) and dexamethasone (6.5 µM) treatments were effective for preventing macrophages from infiltrating the liver as determined by in situ hybridization. Number of independent animals as shown in the parenthesis. (b) Representative whole-mount RNA hybridization for macrophage marker mfap4 showing liver in the dotted region. White dotted liver indicates infiltration, while black dotted liver shows no infiltration. (c) Quantification of the number of infiltrating macrophages either after water or LPS injection in the brain in the control or drug treated conditions as determined by in vivo imaging. (d) Representative single z-plane images from z-stacks that show abundant infiltrating macrophages (arrows) in control treated animals after brain-LPS injection, but no infiltration in dexamethasone or Bay 11–7082 treated larvae. Student’s t-test was used to determine statistical significance.