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Fig. 4

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Publication: Gerrard et al., 2020 - Dynamic changes in the epigenomic landscape regulate human organogenesis and link to developmental disorders
All Figures: Figures for Gerrard et al., 2020

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Fig. 4 Transgenic analysis of H3K27ac regions from human embryonic tissues.

H3K27ac-marked regions were tested in multiple lines of stable transgenic zebrafish (details in Supplementary Data 4; same colour coding of tracks as in Fig. 1). a 231 bp limb enhancer, 502 kb downstream of TBX15, with the corresponding green fluorescent protein (GFP) detection in fin bud at 48 h post fertilisation (hpf). b About 355-bp heart/LV enhancer, 189 kb upstream of HEY2, with the corresponding ventricular GFP detection at 48 hpf. c 1.5 kb palate enhancer, 141 kb downstream of ALX1, with GFP in the developing trabecula and mandible (blue arrows) at 48 hpf. Correlations between the enhancer and transcription of the TF gene are shown for each example. Note the H3K27me3 marks over the gene in each instance in other tissues. *, midbrain GFP expression from the integral enhancer in the reporter vector used as a positive control for transgenesis.

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