Effects of methiothepin on the expression of ER stress-related proteins in ES2 and OV90 cells. (A) Expression of ER stress-related proteins following methiothepin treatment in ES2 and OV90 cells was analyzed through western blotting. (B–E) In order to quantify the expression of (B) GRP78/Bip, (C) p-PERK, (D) ATF4, and (E) CHOP in ES2 and OV90 cells following treatment with methiothepin, the intensity of the band corresponding to each protein was normalized to the β-actin band intensity. The asterisks indicate statistically significant differences compared to the control (* p < 0.05; ** p < 0.01; *** p < 0.001).
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