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Publication: Pollock et al., 2020 - Primary cilia are present on endothelial cells of the hyaloid vasculature but are not required for the development of the blood-retinal barrier
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Fig 1 Endothelial cilia are observed during early development of the hyaloid vasculature, but rapidly decline in number by 3 dpf.

A) Confocal micrograph of the hyaloid vessels (red) of a 3 dpf Tg(flk1:mCherry;bactin2:Arl13b-GFP) embryo. Cilia (green, arrowheads) were observed on the endothelial cells, near the nuclei (DAPI, blue). B) Percentage of hyaloid vessel endothelial cells possessing a primary cilium at 2, 3, and 5 dpf (n > 80 endothelial cells from ≥ 4 eyes per time point). C) Percentage of hyaloid vessel endothelial cells possessing a primary cilium at 2 dpf in ift172 homozygous mutant fish compared to wild-type and heterozygous siblings (n ≥ 4 fish per group). D) Percentage of brain endothelial cells possessing a primary cilium at 2 dpf in ift172 homozygous mutant fish compared to wild-type and heterozygous siblings (n ≥ 3 fish per group). ***p < 0.0005 by Fisher’s exact test. Error bars = SEM.

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