Morphological analysis of microglia in the brain of zebrafish. (A–C) Representative images of different microglial morphology. (A) resting microglia, (B) activated microglia, and (C) dystrophic microglia. Scale bar: 25 μm. (D) Quantitative analysis of the resting, activated, and dystrophic-like cells in the hypothalamus of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5–T2 (D.I.O. zebrafish followed by 3,5–T2), D.I.O. with 3,5–T2 (D.I.O. zebrafish treated with 3,5–T2). Data are expressed as mean ± SE. *** p < 0.0001, ** p < 0.001 compared to the control group. °° p < 0.05 compared to D.I.O.
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