Figure 6

Figure 6

Verification of the suitability of the reaction when normalized with the candidate reference genes ranked as the best for muscle tissue (a,b) and for all tissues grouped (c,d), using muscle gene expression results. As target genes, it was chosen the worst (a) and the third best (b) for muscle. In all the graphics, the Y-axis represents mRNA relative expression, and X-axis indicates the experimental group: non-transgenic and gh-transgenic zebrafish of F0104 lineage. Data are expressed as mean ± standard error of the mean. Asterisk indicate significant difference mean values between groups (n = 7; p < 0.05).