ZFIN Image: Yoshimatsu et al., 2020, Figure 6

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Figure Caption

Figure 6

Calcium Baseline Predicts Dark-Light Responses

(A and B) Whole-eye sagittal view of UV cone SyGCaMP6f in live Tg(opn1sw1:SyGCaMP6f) zebrafish under 3 × 10⁵ photon/s/μm² UV background light (A) and after immunostaining against SyGCaMP6f using anti-GFP antibody (B).

(C) Mean and 95% confidence intervals of the difference between live SyGCaMP6f signal per cone as in (A) and fixed signal as in (B), with red lines indicating regions that were significantly different from zero.

(D) Example mean and individual trial single cone response to 0 photon/s/μm² dark and 6 × 10⁵ photon/s/μm² light steps from a constant brightness UV 3 × 10⁵ photon/s/μm² without and with spectrally broad background light. After five repeats, a 1.5 × 10⁷ photon/s/μm² UV light step was presented to drive calcium to a minimum (right).

(E) Mean and 95% confidence interval of calcium baseline relative to the full dynamic range as indicated, with single datapoints in the back.

(F) Mean ± 1 SD calcium responses to light and dark contrasts with naturalistic RGB background light across all UV cones in specified regions. Traces were shifted and scaled to align the baseline and peak dark response.

(G) Mean and 95% confidence intervals of dark-light index (DLi) with single datapoints in the back.

Acknowledgments

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