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Figure 2—figure supplement 3. Expression of exogenous wild-type Kv3.3 (exoWT) does not significantly alter Purkinje cell excitability.

(A) A representative trace recorded at 5.25 dpf in a Purkinje cell expressing exoWT is shown. (B) Average frequency of simple spikes at 5.25 dpf was calculated from individual 10 s traces for control Purkinje cells and cells expressing exoWT. Each symbol represents a trace. Control data obtained at 5.25 dpf are the same as shown in Figure 1C and are repeated here for comparison to exoWT. For control cells, the mean firing frequency ± SEM was 7.7 ± 0.5 Hz (n = 117). For exo-WT-expressing cells, the mean firing frequency ± SEM was 4.8 ± 0.7 Hz (n = 16). (C) Average frequency of complex spiking is shown for control and exoWT-expressing Purkinje cells at 5.25 dpf. Values, provided as mean ± SEM, were 0.4 ± 0.05 Hz in control cells and 0.3 ± 0.1 Hz in exo-WT expressing cells. Control data obtained at 5.25 dpf are the same as shown in Figure 2—figure supplement 2C and are repeated here for comparison to exoWT.

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