PDIM-deficient M. marinum retain hemolytic activity.
(A) 2-hour hemolysis of sheep red blood cells following incubation with wildtype, ΔESX-1 and ΔmmpL7 M. marinum relative to a Triton X-100 control. Statistical significance was determined by one-way ANOVA with Tukey's post-test. *p < 0.05, ** p < 0.01, otherwise, p > 0.05. Symbols represent matching experimental replicates. Pink circles, green squares, dark purple triangles and light purple diamonds are the first, second, third, and fourth replicates, respectively. (B) ΔESX-1 and ΔmmpL7 M. marinum hemolysis from (A) normalized to wildtype for each of four experiments. p-values, one-sample t-test against a hypothetical mean of 100% Error bars, mean +/- S.E.M.
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