ZFIN Image: Arias-Alpizar et al., 2020, Fig. 5

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Figure Caption

Fig. 5 Real time multi-photon imaging of liposome surface charge switching in situ and in vivo.

a The zebrafish embryo was exposed to UV light between the acquisitions of the camera, resulting in a 95% duty cycle of UV illumination per frame. b Transmission image of the imaging location, emission image overlaid in red. c Maximum intensity projections of two-photon z-stacks (spanning the full width of the embryo) showing DOPC:4 liposome distribution, before and after UV exposure. These images confirm the vessel connected to, and extending dorsally from the PCV, is an ISV. d Time-lapse images of DOPC:4(→3) liposome distribution before and during UV irradiation. In later timeframes, large clusters of liposomes (indicated with white arrowheads) were observed passing through the plane of view in circulation. e Mean fluorescence intensity within the ROI (lumen of the DA, white square in (c, 15 min). Liposome fluorescence intensity immediately decreased upon UV irradiation. High intensity spikes of fluorescence, due to large circulating liposome aggregates passing through the ROI, registered from 5 min after UV irradiation start. Liposomes contained 1 mol% fluorescent lipid probe, DOPE-LR, for visualisation. Scale bars: 50 μm.

Acknowledgments

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