Fig 5

Fig 5

(A) Pair-mating of two Het zebrafish produces a family made up of WT, Het, and Hom siblings in a ~1:2:1 ratio respectively. At six months of age, the transcriptomes of entire brains from four female sibling fish each of WT, Het, and Hom genotypes were analyzed. (B) PCA showing PC1 and PC2 using logCPM values from each sample. The largest source of variability within this data set was clearly the difference between wild type samples and those containing one or two copies of the psen2^S4Ter allele. (C) Volcano plots displaying gene differential expression (DE) p-values versus fold-change. Left: DE genes from comparison of Het and Hom brains. Right: DE genes from comparison of brains of fish possessing the S4Ter allele (i.e. either Het or Hom) versus WT. The dominant nature of the S4Ter mutation is indicated by the relatively restricted differences between Het and Hom transcriptomes compared to S4Ter vs. WT.