ZFIN Image: Deveau et al., 2020, Fig 2

Image

Figure Caption

Fig 2 Live confocal imaging of larval eyes at 4- and 5-days post fertilization (dpf).

(A) A confocal cross section of a wild type (6BP+/+) (N = 4) larval eye shows a ring of cone layer tdTomato fluorescence under control of a trβ2 promoter in the red cones at the outer edge of the retina. (B) A 6BP+1+/- heterozygote maintains (N = 7) trβ2 fluorescence, but the 6BP+1-/- mutant (N = 14) (C) has no trβ2 fluorescence. The 3BP wild type (N = 12) (D), heterozygote (N = 23) (E), and mutant (N = 5) (F) all exhibit trβ2 fluorescence shown through mYFP expressed by a trβ2 promoter. Eyes are imaged from the larval anterior side showing a cross-section ring of cones. Fluorescence in a ring or semi-ring formation indicates the presence of trβ2 activity. Missing sections of the rings are due to image planes cutting through the cornea.

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Acknowledgments

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