Figure 2—figure supplement 1.

Figure 2—figure supplement 1.

(A) Localization of the anti-mCherry nanobody to primary cilia. mIMCD-3 cells were transfected with mNphp3(201)-VHH_LaM-4-eGFP (green) and stained with an acetylated tubulin antibody (cyan, ciliary marker) and DAPI (blue). (B) Localization of the anti-mCherry nanobody and LAPD-mCherry to primary cilia. mIMCD-3 cells were co-transfected with mNphp3(201)-VHH_LaM-4-eGFP (green) and LAPD-mCherry (red) and labeled with DAPI (blue). (C) Localization of the anti-GFP nanobody mNphp3(201)-VHH_enhancer-HA to primary cilia, while LAPD-mCherry resides in the soma. mIMCD-3 cells were co-transfected with mNphp3(201)-VHH_enhancer-HA (green) and LAPD-mCherry (red) and stained with an anti-HA antibody (green) and DAPI (blue). Boxes indicate the position of the magnified view shown at the bottom right. Arrows in different colors indicate the direction and the length of the shift of the respective fluorescence channel. Scale bars: 10 µm.