Her9 mutant retinas display increased apoptosis beginning at 72 hpf. (A–D′) TUNEL staining on cryosections of WT and her9 mutant retinas.Her9 mutant embryos show no significant increase in cell death in the retina at 24 and 48 hpf compared to WT siblings (A–B′). At 72hpf, her9 mutant larvae display increased apoptosis in the GCL, INL, ONL and CMZ (C–C′) that remains elevated in the ONL at 5 dpf (D–D′). Cell counts of TUNEL+ cells (E–G). Note that the data points below the x-axis do not indicate negative numbers, but rather are an attempt by the ggplot2 package to display all the data points (ggplot2 in R: version 3.6.2; https://www.R-project.org/). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; L, lens. Scale bar = 50 µm.
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