CMZ defects in her9 mutants. Immunostaining for PCNA in WT or heterozygous (A,B) and her9 mutant (A′,B′) retinal sections at 72 hpf and 5 dpf; a decrease in the size and fluorescence intensity of the CMZ is apparent in her9 mutants. Area of CMZ (defined as the region of PCNA+ cells directly adjacent to the end of the inner plexiform layer) calculated at 72 hpf and 5 dpf (C). Her9 mutants have a significantly reduced PCNA-positive signal in the peripheral retina. CMZ fluorescence intensity at 72 hpf and 5 dpf (D). GCL, ganglion cell layer; INL, inner nuclear layer; CMZ, ciliary marginal zone. Scale bar = 50 µm.
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