Her9 mutants lack a VBA response and display abnormal visual behavior (OKR). (A,B) Images displaying her9 mutant with significantly darker pigmentation at 5 dpf. (C) Genotyping of the “dark” and “light” larvae revealed that out of 51 individuals classified as “light”, zero were her9−/− (p value < 0.0001, χ2 analysis). Conversely, 12 out of 19 individuals classified as “dark” were her9-−/− (p value = 0.0051). (D) Number of responders and non-responders in the optokinetic response (OKR) assay. (E) Genotyping after OKR revealed that zero of the her9−/− embryos displayed saccades in the OKR (p < 0.0001). Scale bar = 50 µm.
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