IMAGE

Figure 5

ID: ZDB-IMAGE-200713-16
Publication: Angileri et al., 2020 - dnmt1 function is required to maintain retinal stem cells within the ciliary marginal zone of the zebrafish eye
All Figures: Figures for Angileri et al., 2020

Image

Figure Caption

Figure 5

RSCs require dnmt1 function to maintain proliferation. A–F Transverse sections of siblings (A–C) and dnmt1^−/− (D–F) larvae from 3 to 5dpf. Nuclei labeled with DAPI (gray). Cells in S-phase indicated by BrdU incorporation (magenta). Mitotic cells are labeled by a pH3(ser10) antibody (cyan). G Proportions of CMZ cells in S-phase (magenta), G2/M-phase (cyan), or not proliferating (gray) of both siblings and dnmt1^−/−larvae from 3 to 5dpf. H Proportion of CMZ cells labeled with BrdU from 3 to 5dpf between controls and dnmt1^−/−larvae. I Proportion of CMZ cells labeled with pH3 from 3 to 5dpf between controls and dnmt1^−/−larvae. White dotted lines designate CMZ (A–F). Scale bars: 30 μm *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005. Dorsal is up in all images.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.