Figure 4

Figure 4

dnmt1 is required to maintain RSC gene expression. Gene expression shown in whole mount (A, C, E, G, I, K, M, O, Q, S) and transverse cryosections (B, D, F, H, J, L, N, P, R, T) between siblings and dnmt1^−/− larvae. A–Dcol15a1b expression. E–HccnD1 expression. I–Ldnmt1 expression. M–Pcdkn1ca expression. Q–Tatoh7 expression. Numbers in transverse cryosections designate the number of larvae that showed the displayed expression pattern versus the total number of larvae analyzed. Scale bars = 75 mm (whole mount) and 10 μm (transverse sections). Anterior is up in all whole-mounts and dorsal is up for all section images. U qPCR results showing relative gene expression levels of cell cycle genes (ccna2, ccnb1, ccnd1, ccne, cdk1, cdk2, and cdk4), cell arrest genes (caspa, caspb, mdm2, p53, and ripk1), and inflammatory response genes (tnfα and il-1β) in whole 4dpf sibling (white bars) and dnmt1^−/− (gray bars) larvae.