Fig. 4

Fig. 4 USP5 enhances the expression of RIG-I. A) USP5 enhances the protein level of RIG-I. Co-translate USP5 and Vector with RIG-I, respectively. Protein samples were collected after 24 h. Detection of changes in protein levels. B) USP5 enhanced RIG-I protein level in a dose-dependent manner. In FHM cells, USP5 eukaryotic expression plasmid 0ug, 0.5ug, 1ug, 1.5ug and Vector 1.5ug,1ug,0.5ug,0ug were co-transformed with 1ug RIG-I, respectively. After 24 h, the expression levels of RIG-I and USP5 were detected. C) USP5 can activate higher IFNφ1 levels stimulated by Poly (I: C). 1ug USP5 or 1ug Vector was transfected into FHM cells in the presence or absence of poly (I: C). Samples were collected after 24 h. Isolate RNA. qRT-PCR was used to detect the mRNA expression level of IFNφ1. N = 3, P value < 0.05 was marked with *, p < 0.01 was marked with **, p < 0.001 was marked with *** and p < 0.0001 was marked with ****.