ZFIN Image: Zhang et al., 2020, Figure 1

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Figure Caption

Figure 1

(A) Schematic representation for EV generation from cells. (B) EV isolation process by multiple centrifugation. (C) SDS PAGE gel of EVs, showing a thick band with molecular weight of ~200 kD and a few thin bands between 50–200 kD using Coomassie Blue staining. (D) Band-shift assay of DNA, PEI/DNA complexes, and PEI/DNA/EVs complexes, at different N/P ratios (the molar ratio of nitrogen in PEI to phosphor in DNA). In (D) PEI_60kD was used in lanes 3–9 and PEI_25kD was used in lanes 10–15.

Acknowledgments

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