Fig 4

Fig 4

A-C. MA plots (M: log ratio; A: mean average) showing genes that are upregulated (fold change > 1.5 and FDR < 0.05) (red), downregulated (fold change < 1.5 and FDR < 0.05) (blue), or unchanged (gray) in rbm8a mutant embryos compared to WT siblings at 21 hpf (A), magoh mutant embryos compared to WT siblings at 21 hpf (B), and rbm8a mutant embryos compared to WT siblings at 27 hpf (C). rbm8a, magoh, and eif4a3 are labeled in each plot with label colors signifying no change (gray) or downregulation (blue). D. Venn diagrams showing the overlap between genes that are upregulated (top) and downregulated (bottom) in rbm8a mutant embryos at 21 hpf (left) and magoh mutant embryos at 21 hpf (right). Hypergeometric test p-values are below each comparison. E. Venn diagrams as in (D) comparing upregulated and downregulated genes in rbm8a at 21 (left) and 27 hpf (right). F. PANTHER14.0 [86] gene ontology (GO) term overrepresentation analysis of genes downregulated in rbm8a and magoh mutant embryos at indicated times. All significant terms (Benjamini-Hochberg corrected p-value < 0.05) are shown for each set. The number of genes in each term is indicated at the right of each bar.