Fig 2

Fig 2

(A) Fins from wildtype (top) and mdka^mi5001 (bottom) at 0, 4, 6, 19, and 32 dpa. Red lines indicate the level of the amputation plane. In wildtype animals, the structure of the bony fin rays and pigmentation recover completely. Compared to age-matched group of wildtype, pre-amputated fin in the mdka^mi5001 is smaller in size (wildtype: 51.3 ± 7.1 μm²; mdka^mi5001: 34.3 ± 4.7 μm², p<0.0001, Student’s t-test). In the mutants, regenerated fins display abnormal shape and mis-patterned melanophore pigmentation (red arrow). Scale bar equals 400 μm. (B) Graph illustrating the average percent outgrowth through 32 dpa, calculated from the ratio of the areas of pre- and post-amputation fins. Wildtype fins recover to 100% of pre-amputation levels by 32 dpa. The mutant animals show a significant reduction of fin outgrowth at 4 dpa. At the subsequent time points, there is no statistically significant difference in the fin outgrowth between wildtype and mdka^mi5001. (C) Graph illustrating the percent fin outgrowth at 4 dpa in wildtype and mdka^mi5001. Student’s t-test, p = 0.0105. n = 9 wildtype and 8 mutants.