ZFIN Image: Sun et al., 2020, Fig. 5

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Figure Caption

Fig. 5 Identifying β-catenin as an ADNP interacting protein.

a Schematic representation showing the experimental design of IP in combination mass spectrometry assay (left) and a list of representative ADNP interacting proteins (right). b Co-IP data for endogenous ADNP and β-catenin in ESCs. Up: IP ADNP followed by WB β-catenin; bottom: IP β-catenin followed by WB ADNP. c Co-IP data for endogenous ADNP and β-catenin of day 3 ESC-derived neurospheres. d Co-IP data for FLAG-ADNP and β-catenin in 3×FLAG-ADNP overexpressing Adnp−/− ESCs. e Schematic representation of the full-length and the truncated ADNP mutants. f IP of FLAG-ADNP-Nter (1–685) and Myc-β-catenin in HEK293T cells. g IP of in vitro synthesized HA-β-catenin and FLAG-ADNP. h Co-IP of FLAG-ADNP-NAP and HA-β-catenin in HEK293T cells. i Schematic representation of the full-length and the truncated β-catenin mutants. j Co-IP of FLAG-β-catenin (151–666) and ADNP in HEK293T cells. k Co-localization of ADNP and β-catenin in day 19 ESC-derived neuronal cell cultures. All WB and IF experiments were repeated at least two times. Similar results were obtained and shown are the representative images.

Acknowledgments

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