ZFIN Image: Loh et al., 2020, Fig. 5

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Figure Caption

Fig. 5 Effects of <italic>RSPO3</italic>-KD on canonical and non-canonical WNT signalling in SC abdominal and gluteal APs.

a Western blots of canonical WNT signalling markers and b qRT-PCR of AXIN2, a WNT/β-catenin target gene, in control (shCON) and RSPO3-KD (shRSPO3) imAbdo (n = 7 experiments) and imGlut APs (n = 9 experiments). c qRT-PCR of AXIN2 in shCON and shRSPO3 1° APs. d RSPO3-KD in imAbdo versus imGlut cells has differential effects on TOPflash promoter activity [W3A (50), 20-h treatment with 50 ng ml^-1 rhWNT3A] (n = 6 replicates/treatment). e Western blots of non-canonical WNT signalling markers in shCON and shRSPO3 imAbdo and imGlut APs. f Protein densitometry for pJNK/tJNK and pCAMK2A/tCAMK2 are shown relative to shCON levels. n = 4 independent experiments. Histogram data are means ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001. Statistical significance was assessed by two-tailed paired (b, c, f) and unpaired (d) Student’s t-test. Source data are provided as a Source Data file.

Acknowledgments

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