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Fig. s1 Control assessment of SCP labelling in Plp1CreERT2 mouse embryos (A-B) Genetic tracing of Plp1CreERT2;R26RYFP/+ embryos from E11.5 to E12.5, in combination with immunodetection of SOX10 (to visualize SCPs) and of TUJ1 (to visualize the nerves) revealed overlap between YFP+ cells and SCPs localized along the nerves in both facial region (A, nasal area shown) and the trunk (B, spine area shown), confirming genetic labeling of the peripheral glial cells with Plp1CreERT2 at this time. (C-D) One-day tracing of the Plp1CreERT2;R26RYFP/+ embryos from either E12.5 to E13.5 (C) or E15.5 to E16.5 (D) revealed comparable Cre recombination efficiency during the two tracing periods. Numbers show percentage of double Cre+YFP+ cells among Cre+. (E) Tracing in Plp1CreERT2;R26RConfetti/Confetti embryos from E12.5 to E13.5 revealed overlap between immunostaining for the CRE protein and the SOX10 marker for SCPs along the TUJ1+ nerves. (F) Immunodetection of confetti proteins with anti-GFP antibody revealed overlap between the Confetti+ cells and SOX10+ SCPs along the TUJ1+ nerves. (G-H) Tracing of Plp1CreERT2;R26RConfetti/Confetti embryos from E12.5 to E13.5 revealed no overlap between the Cre antibody staining (G) or retrieved Confetti signal (H) and the PDGFRa+ mesenchymal cells.