ZFIN Image: Tie et al., 2019, Fig. 2

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Figure Caption

Fig. 2 Il6 signaling is required for HSC-independent neutrophil development, but is not indispensable for the development of HSC-independent macrophages and primitive erythrocytes.

a Representative images of Std, Il6r, and Il6 morphants detected by WISH for mpx and l-plastin expression at 28 hpf. The white block denotes the posterior blood island (PBI) region, and the black block denotes the PBI and trunk regions. b Qualitative phenotype distribution of embryos from a scored with medium (normal) and low (down) mpx and l-plastin expression, respectively. Medium, white bar; low, gray bar. c Confocal tracking of HSC-independent macrophages in mpeg1:GFP transgenic embryos injected with Std, Il6r, and Il6 MOs at 32–36 hpf. d Enumeration of mpeg1⁺ macrophages cells in the yolk sac, PBI, and trunk regions from c. Bars represent the means ± SEM of Std (n = 12), Il6r (n = 12), and Il6 (n = 12) morphants. n.s., Not significant. e Representative images of Std, Il6r, and Il6 morphants interrogated by WISH for gata1a expression at 24 hpf. f Qualitative phenotype distribution of embryos from e scored as in b

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