ZFIN Image: Santos-Ledo et al., 2020, Fig 4

Image

Figure Caption

Fig 4 <italic>jnk1a/b</italic> mutants.

Sequences of genomic DNA for jnk1a(A) and jnk1b(B) showing targeting sequence for CRISPR-Cas9 guide RNA; deletions produced; translated peptide sequence (* = stop); and restriction enzymes that identify mutants. Both jnk1a and jnk1b mutations disrupt sequence within exon 6 and predicted to lead to loss of all alternatively spliced transcripts by nonsense-mediated decay. (C) Maternal zygotic (MZ) jnk1a, MZjnk1b and MZjnk1a/ MZjnk1b mutants all appear grossly normal and are fertile. (D) RT-PCR using primers specific for jnk1a and jnk1b exon 7 and 8 show nonsense-mediated decay in MZjnk1a and MZjnk1b mutants. * = p<0.05, ** = p<0.01, **** = p<0.0001. n = 3 (3 separate clutches of 40 embryos obtained from different pairs of fish). Scale bar 1mm.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.