ZFIN Image: Albadri et al., 2020, Figure 5

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Figure Caption

Figure 5

Barhl1a and Ptf1a reporter expressions are mutually exclusive. (A–B) Confocal imaging through the retina of a 50 hpf Tg(barhl1a:GFP;ptf1a:dsRed) embryo fixed and counterstained with DAPI (blue). Each image represents a single Z-stack in frontal view. Anterior is to the top. (B) always represents a higher magnification of (A). GFP-positive (green) cells are detected in the GCL whilst DsRed-positive cells (red) are detected in the amacrine (am) cells and horizontal (ho) cells of the inner nuclear layer (INL). White arrows point at DsRed-positive amacrine cells displaced in the GCL; which are always GFP-negative. Asterisks highlight the GFP-negative cells in the GCL; which are also DsRed-negative (B). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; am, amacrine cells; ho, horizontal cells. Scale bars: (A) 50 µm, (B) 20 µm.

Acknowledgments

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