ZFIN Image: Cheng et al., 2004, Figure 7

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Figure Caption

Figure 7 Sequence Determinants Conferring EGF-CFC Dependence

Synthetic mRNAs (200 pg) encoding ActivinβB with single or double region substitutions from Sqt were injected into wild-type and MZoep embryos. gsc and ntl mRNA expression is shown at shield stage; animal pole views are dorsal to the right. Schematic is not drawn to scale. HA indicates a hemagglutinin epitope tag. Note that Act^Sqt[loop^β^8] containing the loop-β8 region of Sqt is inactive in both wild-type and MZoep embryos. In Act^{Sqt[Finger1-loop}^β^8], the additional substitution of Sqt Finger 1 region relieves the inhibitory presence of the Sqt loop-β8 region. Similar to Sqt, Act^{Sqt[Finger1-loop}^β^8] can induce ectopic gsc and ntl in wild-type, but not in MZoep embryos. Western blot analysis indicates that these chimeric constructs produce stable ligands (data not shown).

Acknowledgments

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