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Fig. 7
Endothelial uptake of NPs. Illustration of the steps undertaken by the endothelial analysis MACRO. A) Zebrafish embryos injected with 99 nm PEGylated liposomes with biotin (white) and fluorescent macrophages (red) as well as endothelium (green) are used for the analysis. B) NPs and C) macrophages are first thresholded. D) The NP signals in macrophages regions are deleted. Subsequently the signal of the endothelium is E) thresholded and F) overlapped to the residual NP measuring their fluorescence. The obtained values are normalized by the fluorescence of the whole zebrafish (panel (F), lower image).
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