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Fig. 4 Both of the major ENS neuronal subtypes are reduced in uhrf1 mutants. Confocal images of dissected intestines from wildtypes (A,C,E,G) and uhrf1 mutants (B,D,F,H) stained for expression of the pan-neuronal marker Elavl, and either nNOS or 5-HT. In both proximal (B,F) and distal (D,H) intestine, uhrf1 mutants have fewer enteric neurons than wildtype siblings at 5 dpf (proximal A,E; distal C,G) as quantified in (I). Both nNOS and 5-HT expressing ENS neuronal subtypes show similar reductions at 5 dpf [Elavl (green), nNOS or 5-HT (magenta)] as quantified in (J,K). Arrows point to examples of nNOS or 5-HT positive neurons. (I) Quantification of Elavl positive cells per 200 μm of proximal or distal intestine in wildtype (blue) or mutant (red) [wildtype n = 30 (proximal), n = 29 (distal); uhrf1−/− n = 29 (proximal), n = 30 (distal)]. (J–K) Quantification of Elavl and nNOS (J) and 5-HT (K) positive cells per 200 μm proximal or distal intestine in wildtype (blue) and mutant (red) [nNOS: wildtype n = 11 (proximal), n = 15 (distal); uhrf1−/− n = 17 (proximal), n = 17 (distal); 5-HT: wildtype n = 17 (proximal), n = 14 (distal); uhrf1−/− n = 15 (proximal), n = 13 (distal)]. Asterisk in B indicates autofluorescent background common to uhrf1 mutant intestines. Scale bar = 50 μm in A-H. Unpaired t-test: **** = p < 0.0001; ** = p < 0.01.
Reprinted from Developmental Biology, 455, Ganz, J., Melancon, E., Wilson, C., Amores, A., Batzel, P., Strader, M., Braasch, I., Diba, P., Kuhlman, J.A., Postlethwait, J.H., Eisen, J.S., Epigenetic factors Dnmt1 and Uhrf1 coordinate intestinal development, 473-484, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.