IMAGE

Fig. 3

ID

ZDB-IMAGE-200514-30

Genes

EGFP

Publication

Paatero et al., 2018 - Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

All Figures

Figures for Paatero et al., 2018

Image

Figure Caption

Fig. 3 Oscillatory F-actin dynamics during remodeling of cell–cell junctions. a, b Still images from a movie (Supplementary Movie 4) showing JBL formation in the dorsal aorta of an EGFP-UCHD expressing 2dpf embryo (Tg(fli:Gal4ffubs3; UAS:EGFP-UCHDubs18)), shown in inversed contrast. b A magnification of the inset in a and the red dashed line indicates the site for kymograph in c. Arrows point to a JBL, seen as a local thickening of the junction. c Kymograph across the junction. Solid arrow denotes forward movement and dashed arrow backward movement of the junction. d Intensity plotting of a EGFP-UCHD JBL kymograph. e Scatter plot of the relative EGFP-UCHD intensity during forward and backward movements (n = 20 events, 4 movies). EGFP-UCHD intensity value in a forward movement was divided with intensity value during subsequent reverse movement. Non-parametric one sample Wilcoxon signed rank test was used as statistical test. Scale bars 5 µm

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.